eTOPIX_Unique ZIKV, BAFF, ADAM17 and ANGPT2 Specific Functional Antibodies

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eTOPIX

Functional Antibodies 

For In Vivo Studies


 
 

UNIQUE from AdipoGen:

NEW ZIKV E Protein Neutralizing Antibody
 
ZIKV E Protein (EIII domain), mAb (rec.) (neutralizing) (ZKA64) (PF) AG-27B-6004PF
 


Recognizes and binds specifically to the EDIII domain of the Zika Virus envelope protein (ZIKV E). No cross-reaction with Dengue virus envelope protein (DENV E).

Functional Application (Neutralizing): Neutralizes Zika Virus (ZIKV) with an IC50 of ~93ng/ml. Enhances ZIKV infection in non-permissive K562 cells at a broad range of concentrations (not above 1µg/ml).


LIT: Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection: K. Stettler, et al.; Science 353, 823 (2016)

 

Unique Antibody to Deplete B Cells In Vivo
 
BAFF (mouse), mAb (blocking) (Sandy-2)
BAFF (mouse), mAb (blocking) (Sandy-2) (PF)
AG-20B-0063
AG-20B-0063PF


Sandy-2 is highly potent in blocking mouse BAFF in vivo. Induces B cell depletion and generates a phenotype similar to that observed in BAFF-/- mice.

LIT: Antibodies that block or activate mouse B cell activating factor of the TNF family (BAFF) respectively induce B cell depletion or B cell hyperplasia: C. Kowalczyk-Quintas, et al.; J. Biol. Chem. 291, 19826 (2016)
 


anti-BAFF (mouse), mAb (Sandy-2) (Prod. No. AG-20B-0063) blocks the action of endogenous BAFF in vivo.
 

anti-BAFF mAb Sandy-2, Blocking

Method: Wild type C57BL/6 mice were treated at day 0 (single administration) with anti-BAFF (mouse), mAb (Sandy-2) (at 2mg/kg). Lymph nodes were prepared at week 2 and analyzed by FACS for the presence of T (CD3) and B (CD19) cells. Untreated BAFF WT and KO mice were analyzed in parallel.
 



Potent  Angiopoietin-2 Blocking Antibody
 
Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1)
Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (PF)
AG-27B-0016
AG-27B-0016PF


Recognizes human & mouse angiopoietin-2. Does not detect human angiopoietin-1.

Functional Application (Blocking):
Inhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50* = 50-60ng/ml (for 10ng/ml of mouse Angiopoietin-2).  Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50* = 8-12ng/ml (for 10ng/ml of human Angiopoietin-2).

*ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2).

LIT: Elevated Angiopoietin-2 Level in Patients With Continuous-Flow Left Ventricular Assist Devices Leads to Altered Angiogenesis and Is Associated With Higher Nonsurgical Bleeding: C.E. Tabit, et al.; Circulation 134, 141 (2016)

 

Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by anti-ANGPT2, mAb (rec.) (Angy-2-1). 

Method: 
Tie-2 (human):Fc was coated on an ELISA plate at 1µg/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) (Prod. No. AG-27B-0016PF) or an unrelated mAb (recombinant) (Control) were added (starting at 40µg/ml with a twofold serial dilution) together with 20ng/µl of Angiopoietin-2 (human) (Prod. No. AG-40B-0114). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).
 

anti-ANGPT2 Angy-2-1 mAb_Blocking


NEW ADAM17 Blocking Antibody
 
ADAM17 (human), mAb (rec.) (blocking) (D1(A12)) (PF)
ADAM17 (human), mAb (rec.) (blocking) (D1(A12)) (Fab Fragment) (His) (PF)
AG-27B-6000PF
AG-27B-6003PF
 


Recognizes the catalytic and non-catalytic domain of human ADAM17 (TACE) through its variable light (VL) domain and variable heavy (VH) domain, respectively. Does not bind recombinant mouse ADAM17 ectodomain.

Functional Application (Blocking): Inhibits ADAM17 activity at 15μg/ml (200nM).


LIT: Cross-domain inhibition of TACE ectodomain: C.J. Tape, et al.; PNAS 108, 5578 (2011)

 

Figure: anti-ADAM17 (human), mAb (rec.) (blocking) (D1(A12)) inhibits constitutive shedding of TNF-α from IGROV1 (human ovarian cancer cell line) into culture medium. Medium was collected after 48 hours of incubation with or without IgGs at 200nM.






 


 
anti-ADAM17 mAb_Blocking


Other Fully Validated Functional ANTIBODIES from AdipoGen:
 

Selected Functional Antibodies
 
PRODUCTNAME PID
APRIL (mouse), mAb (rec.) (blocking) (Apry-1-1) (PF) AG-27B-0001PF
CD40 (mouse), mAb (activating) (FGK45) (PF) AG-20B-0036PF
CD40L (human), mAb (rec.) (blocking) (hu5c8) (PF) AG-27B-6002PF
Fibronectin (EDA), mAb (blocking) (IST-9) (PF) AG-20B-6001PF
IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) (PF) AG-27B-0013PF
LAG-3 (human), mAb (blocking) (17B4) (preservative free) AG-20B-0012PF
Lewisy/b hapten (human), mAb (SC104) (preservative free) AG-27B-6002PF
Netrin-1 (human), mAb (rec.) (blocking) (2F5) (PF) AG-27B-0018PF
Neutrophils (mouse), mAb (blocking) (Nimp-R14) (PF) AG-20B-0043PF
OX40L (human), mAb (rec.) (blocking) (R4930) (PF) AG-27B-6001PF


*PF = Preservative Free
 
Functional Antibodies Brochure
 

Includes selected Functional Antibodies from AdipoGen Life Sciences and Ancell Corporation.









 
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Functional Antibodies Brochure


 
Key Research Areas:
INFLAMMASOMES | INFLAMMATION
CANCER | OBESITY & DIABETES 
IMMUNITY | STEM CELL BIOLOGY
IMMUNO-ONCOLOGY
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    PROTEINS | SMALL MOLECULES
    FLUORESCENT DYES & PROBES

 
 
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